首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：人α-黑色素细胞刺激素(α-MSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的α-MSH含量。α - MSH即α - 促黑素细胞激素，是一种内源性神经肽。它在调节皮肤色素沉着、能量代谢、炎症反应等方面发挥重要作用。研究机制主要涉及与黑素皮质素受体结合，激活下游信号通路，进而对多种生理功能产生影响。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品广泛应用于基础科研领域，如探究α-MSH在代谢综合征动物模型中的表达特征、评估体外细胞刺激实验的激素分泌水平，或解析皮肤黑色素生成调控机制等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：alpha-melanocyte stimulating hormone, alpha-MSH
缩写：α-MSH
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-95 | ||||
| EDTA plasma (n=4) | 94 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.0812.1812.1312.00051.2561.1981.2271.0962.50.6550.6350.6450.5141.250.4060.4160.4110.2800.6250.2600.2700.2650.1340.3120.2200.2190.2200.0890.1560.1870.1820.1850.05400.1320.1300.131 | ||||||
×
