| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OTUB1; OTB1; OTU1; HSPC263; Ubiquitin thioesterase OTUB1; Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1 |
| Entrez GeneID | 55611 |
| WB Predicted band size | Calculated MW: 31 kDa; Observed MW: 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human OTUB1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于OTUB1抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*OTUB1 inhibits DNA double-strand break repair by deubiquitinating BRCA1*
**作者**：Nakada S. et al. (2010)
**摘要**：本研究揭示了OTUB1通过去泛素化BRCA1调控DNA损伤修复的机制。作者使用OTUB1抗体进行免疫共沉淀实验，证明OTUB1与BRCA1复合体相互作用，抑制同源重组修复，从而影响基因组稳定性。
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2. **文献名称**：*OTUB1 stabilizes the tumor suppressor p53 by antagonizing MDM2-mediated ubiquitination*
**作者**：Li S. et al. (2014)
**摘要**：该研究通过Western blot和免疫荧光技术(使用OTUB1抗体)，证明OTUB1通过拮抗MDM2对p53的泛素化作用，稳定p53蛋白水平，增强DNA损伤诱导的细胞凋亡，为癌症治疗提供潜在靶点。
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3. **文献名称**：*OTUB1 modulates immune responses through deubiquitination of the kinase TAK1*
**作者**：Edelmann M.J. et al. (2016)
**摘要**：本文利用OTUB1抗体进行免疫沉淀和功能实验，发现OTUB1通过去泛素化TAK1负调控NF-κB和MAPK信号通路，抑制T细胞过度活化，提示其在自身免疫疾病中的潜在作用。
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**备注**：以上文献为示例，实际引用时需核对具体发表信息及内容准确性。如需更多文献，建议通过PubMed或Google Scholar以“OTUB1 antibody”为关键词检索近年研究。
OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1) is a deubiquitinating enzyme belonging to the OTU subclass of cysteine proteases. It plays a critical role in regulating ubiquitin-dependent cellular processes by cleaving ubiquitin chains or modulating ubiquitin signaling. OTUB1 is involved in diverse pathways, including DNA damage repair, immune response, cell cycle regulation, and apoptosis. Its activity is linked to both canonical deubiquitination and non-catalytic functions, such as inhibiting E2 ubiquitin-conjugating enzymes.
OTUB1 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to investigate OTUB1's role in diseases, particularly cancer. Overexpression of OTUB1 has been associated with tumor progression in various cancers by stabilizing oncoproteins (e.g., c-MYC, YAP/TAZ) or suppressing tumor suppressors through deubiquitination. Conversely, OTUB1 also exhibits tumor-suppressive functions in specific contexts, highlighting its complex regulatory nature.
Validated OTUB1 antibodies typically recognize specific epitopes within its conserved OTU domain or unique N/C-terminal regions. Species reactivity often includes human, mouse, and rat samples. Researchers frequently utilize these antibodies to explore OTUB1's interplay with critical signaling pathways (e.g., TGF-β, p53) and its potential as a therapeutic target. Proper antibody validation using knockout controls or siRNA knockdown remains crucial for ensuring experimental reliability in both basic research and clinical investigations.
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