| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Grp170; HSP12A; Hyou1; Orp150 |
| Entrez GeneID | 10525 |
| WB Predicted band size | Calculated MW: 111 kDa; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ORP150 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ORP150抗体的3篇参考文献示例(注：文献信息为示例性概括，仅供参考)：
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1. **文献名称**：*Oxygen-regulated protein 150 (ORP150) suppresses hypoxia-induced apoptotic cell death via modulation of mitochondrial function*
**作者**：Ozawa K, et al.
**摘要**：本研究通过免疫组化和Western blot分析，利用ORP150抗体探究其在心肌缺血模型中的表达变化，发现ORP150通过调控线粒体功能减轻缺氧诱导的细胞凋亡。
2. **文献名称**：*Role of ORP150 in promoting tumor angiogenesis under endoplasmic reticulum stress*
**作者**：Miura T, et al.
**摘要**：通过ORP150抗体的免疫荧光染色和基因沉默实验，揭示ORP150在内质网应激条件下通过稳定HIF-1α促进肿瘤血管生成的作用机制。
3. **文献名称**：*ORP150/HYOU1 as a novel biomarker for Alzheimer's disease: Evidence from cerebrospinal fluid analysis*
**作者**：Bando Y, et al.
**摘要**：采用ORP150特异性抗体检测阿尔茨海默病患者脑脊液样本，发现ORP150水平与神经退行性病变严重程度相关，提示其作为潜在生物标志物的价值。
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**备注**：以上文献信息为示例，实际引用时需核实真实来源及细节。建议通过PubMed、Web of Science等数据库，以“ORP150 antibody”“HYOU1 (gene name)”“oxygen-regulated protein”等关键词检索最新研究。
ORP150 (Oxygen-Regulated Protein 150), also known as GRP170 or HYOU1. is a stress-inducible molecular chaperone belonging to the heat shock protein 70 (HSP70) family. It was first identified in the 1990s as a protein upregulated under hypoxic conditions, playing a critical role in cellular adaptation to low oxygen tension and endoplasmic reticulum (ER) stress. Structurally, ORP150 contains an N-terminal ATPase domain and a C-terminal substrate-binding domain, enabling its participation in protein folding, quality control, and the unfolded protein response (UPR).
This 150 kDa protein is predominantly localized in the ER and is essential for maintaining cellular homeostasis during stress by stabilizing nascent proteins, preventing aggregation, and aiding in the degradation of misfolded proteins. Its expression is regulated by hypoxia-inducible factor 1 (HIF-1α) and other stress-sensitive signaling pathways.
ORP150 antibodies are widely used in research to study its role in diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and ischemic injuries. Elevated ORP150 levels are associated with tumor progression, chemoresistance, and poor prognosis in certain cancers, while its neuroprotective effects in stroke models highlight its dual context-dependent roles. These antibodies enable detection via Western blot, immunohistochemistry, and immunofluorescence, aiding mechanistic insights into ER stress-related pathologies and therapeutic targeting.
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