首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠α谷胱甘肽S转移酶(α-GST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的Gsta1含量。试剂盒检测范围为0.156 mU/mL-10 mU/mL，常用于评估药物毒性、氧化应激及肝脏病理机制等研究。支持科研实验中多种生物样本的批量检测，适用于构建肝损伤模型、药物代谢动力学分析或环境毒理学研究等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gsta1 ELISA Kit; Glutathione S-transferase alpha-1 ELISA Kit; EC 2.5.1.18 ELISA Kit; 13-hydroperoxyoctadecadienoate peroxidase ELISA Kit; EC 1.11.1.- ELISA Kit; Androst-5-ene-3,17-dione isomerase ELISA Kit; EC 5.3.3.- ELISA Kit; GST 1-1 ELISA Kit; GST 1a-1a ELISA Kit; GST A1-1 ELISA Kit; GST B ELISA Kit; Glutathione S-transferase Ya-1 ELISA Kit; GST Ya1 ELISA Kit; Ligandin ELISA Kit
缩写：Gsta1
Uniprot No.：P00502
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 mU/mL-10 mU/mL
灵敏度：0.039 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of rat α-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 82-89 | ||||
| EDTA plasma (n=4) | 97 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected 102.5882.5992.5942.394 51.8841.8991.8921.692 2.51.2651.2871.2761.076 1.250.8520.8980.8750.675 0.6250.5880.5980.5930.393 0.3120.4520.4690.4610.261 0.1560.3740.3970.3860.186 00.1990.2010.200 | ||||||
货期：3-5 working days
×
