| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ORP1; OSBP8; OSBPL1; OSBPL1B |
| Entrez GeneID | 114876 |
| WB Predicted band size | Calculated MW: 108 kDa; Observed MW: 109,50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ORP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ORP1抗体的3篇文献示例(注：文献为虚构示例，实际文献需通过数据库查询确认)：
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1. **文献名称**: *ORP1 regulates lysosomal positioning and cholesterol transport through interaction with VAPA/VAPB*
**作者**: Vihervaara T, et al.
**摘要**: 本研究利用ORP1特异性抗体进行免疫荧光和Western blot实验，揭示了ORP1通过与内质网膜蛋白VAPA/VAPB结合，调控溶酶体分布和胆固醇转运的分子机制，为溶酶体功能异常相关疾病提供了新见解。
2. **文献名称**: *OSBP-related protein 1 (ORP1) mediates endosomal trafficking via Rab7 interaction*
**作者**: Zhou Y, et al.
**摘要**: 通过ORP1抗体的免疫共沉淀实验，作者发现ORP1与Rab7 GTP酶相互作用，调控晚期内体向溶酶体的运输过程，并证明该过程依赖于ORP1的脂质结合结构域。
3. **文献名称**: *Role of ORP1 in cellular lipid homeostasis and neurodegenerative disorders*
**作者**: Johansson M, et al.
**摘要**: 该研究利用ORP1抗体在小鼠脑组织中的免疫组化分析，发现ORP1在神经元中的高表达及其与脂筏形成的关联，提示其可能参与阿尔茨海默病的病理进程。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词：**ORP1 antibody**, **OSBPL1A**, **oxysterol-binding protein-related protein 1**，并筛选实验方法中明确使用ORP1抗体的研究。
The ORP1 antibody is a crucial tool for studying the Oxysterol-binding protein-related protein 1 (ORP1), a member of the OSBP-related proteins (ORPs) family involved in lipid metabolism, intracellular signaling, and organelle interactions. ORP1 regulates cholesterol transport, endosomal-lysosomal system dynamics, and cellular responses to lipid imbalances. It localizes to late endosomes/lysosomes and interacts with RAB7 and VAP proteins to mediate membrane contacts, influencing organelle positioning and cholesterol distribution. Dysregulation of ORP1 is linked to diseases like cancer, neurodegenerative disorders, and lysosomal storage diseases.
The ORP1 antibody enables detection and localization of ORP1 in various experimental models, facilitating research into its roles in lipid homeostasis and disease mechanisms. Commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation, this antibody helps elucidate ORP1’s interaction partners, post-translational modifications, and responses to lipid stress. Studies using ORP1 antibodies have revealed its involvement in autophagy, lysosomal function, and cancer cell survival, highlighting its potential as a therapeutic target.
Developed against specific epitopes (e.g., N-terminal or C-terminal regions), ORP1 antibodies vary in species reactivity (human, mouse, rat) and applications. Validation in knockout controls ensures specificity, making them essential for mechanistic studies in lipid biology and related pathologies.
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