首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人α谷胱甘肽S转移酶(α-GST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的α-GST含量。人α谷胱甘肽S转移酶（α-GST）是一种重要的解毒酶，参与细胞内有害物质的代谢。其活性与肝毒性相关，在七氟烷肝毒性研究中，α-GST活性升高与特定基因突变有关，提示其在药物代谢和毒性反应中的关键作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究肝脏功能机制、药物代谢毒性评估、氧化应激相关信号通路分析等科研领域，为探索疾病模型或环境暴露下的细胞保护机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Glutathione S alkyltransferase A1 ELISA Kit; Glutathione S aryltransferase A1 ELISA Kit; Glutathione S transferase 2 ELISA Kit; Glutathione S transferase A1 ELISA Kit; Glutathione S transferase alpha 1 ELISA Kit; Glutathione S transferase Ha subunit 1 ELISA Kit; Glutathione S-transferase A1 ELISA Kit; GST class-alpha member 1 ELISA Kit; GST epsilon ELISA Kit; GST HA subunit 1 ELISA Kit; GST; class alpha; 1 ELISA Kit; GST-epsilon ELISA Kit; GST2 ELISA Kit; GSTA1 1 ELISA Kit; GSTA1 ELISA Kit; GSTA1-1 ELISA Kit; GSTA1_HUMAN ELISA Kit; GTH1 ELISA Kit; HA subunit 1 ELISA Kit; MGC131939 ELISA Kit; OTTHUMP00000016611 ELISA Kit; S (hydroxyalkyl)glutathione lyase A1 ELISA Kit
缩写：α-GST
Uniprot No.：P08263
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human α-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-109 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 81-90 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human α-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 88 | 84-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7162.6292.6732.55952.0712.0022.0371.9232.51.4821.5671.5251.4111.250.9040.9610.9330.8190.6250.5890.6230.6060.4920.3120.3650.3840.3750.2610.1560.2590.2630.2610.14700.1130.1150.114 | ||||||
货期：3-5 working days
×
