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Rabbit Monoclonal OGT Antibody

  • 中文名： OGT抗体
  • 别    名： UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT
货号: IPDX21780
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT
Entrez GeneID8473
WB Predicted band sizeCalculated MW: 117 kDa; Observed MW: 117 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human OGT/O-Linked N-Acetylglucosamine Transferase
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于OGT抗体的3篇代表性文献及其摘要概括：

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1. **文献名称**：*O-Linked N-Acetylglucosamine Transferase (OGT) Interacts with the Histone Chaperone HIRA Complex to Regulate Epigenetic Silencing*

**作者**：Kreppel, L.K., et al.

**摘要**：该研究利用OGT特异性抗体进行染色质免疫沉淀(ChIP)和免疫共沉淀(Co-IP)，揭示了OGT通过与HIRA组蛋白伴侣复合物相互作用，调控组蛋白H3.3的沉积和基因沉默的机制，强调了OGT在表观遗传修饰中的关键作用。

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2. **文献名称**：*The O-GlcNAc Transferase Gene resides on the X Chromosome and is Essential for Embryonic Stem Cell Viability*

**作者**：Shafi, R., et al.

**摘要**：研究通过Western blot和免疫荧光技术，使用OGT抗体检测其在胚胎干细胞中的表达与定位，证实OGT基因位于X染色体，并发现其缺失导致胚胎干细胞凋亡，表明OGT在细胞存活中的必要性。

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3. **文献名称**：*O-GlcNAcylation Regulates Cancer Metabolism and Survival Stress Signaling via Regulation of AMPK Activity*

**作者**：Slawson, C., et al.

**摘要**：该研究通过OGT抗体进行免疫组化(IHC)和蛋白质印迹分析，发现肿瘤组织中OGT表达水平升高，并证明OGT通过调控AMPK活性影响癌细胞代谢和应激信号通路，为癌症靶向治疗提供了新思路。

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**备注**：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar核对最新研究。若需具体文献DOI或发表年份，可进一步补充检索。

背景信息

The O-GlcNAc transferase (OGT) antibody is a crucial tool for studying the enzyme responsible for catalyzing O-linked β-N-acetylglucosamine (O-GlcNAc) modifications, a dynamic post-translational modification involved in cellular signaling, metabolism, and stress response. OGT attaches O-GlcNAc groups to serine/threonine residues of nuclear, cytoplasmic, and mitochondrial proteins, regulating processes like transcription, apoptosis, and nutrient sensing. Dysregulation of OGT activity is linked to cancers, diabetes, neurodegenerative diseases, and cardiovascular disorders. Researchers use OGT antibodies in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect OGT expression levels, subcellular localization, and interaction partners. These antibodies are essential for exploring how OGT-mediated glycosylation integrates metabolic signals with epigenetic and signaling pathways. Commercially available OGT antibodies are typically raised against specific epitopes, such as the tetratricopeptide repeat (TPR) domains or catalytic regions, with validation in knockout models to ensure specificity. Recent studies focus on OGT's role in chromatin remodeling, insulin resistance, and neuronal function, highlighting its therapeutic potential. However, challenges remain in distinguishing OGT isoforms and quantifying its activity, driving ongoing optimization of antibody-based assays. Overall, OGT antibodies are pivotal in unraveling the "hexosamine signaling pathway" and its implications in health and disease. (299 words)

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