| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DmNXF1; MEX67; Mex67p; Mvb1; NXF1; Sbr; TAP |
| Entrez GeneID | 10482 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NXF1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与NXF1抗体相关的研究文献摘要概览：
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1. **文献名称**：*The human Tap nuclear RNA export factor contains a novel transportin-dependent nuclear localization signal that lacks a sequence requirement*
**作者**：Katahira, J., et al.
**摘要**：该研究利用NXF1(Tap)抗体通过免疫荧光和免疫沉淀技术，揭示了NXF1蛋白的核定位机制，证明其依赖于Transportin蛋白而非经典核定位信号，为mRNA出核运输的分子机制提供了新见解。
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2. **文献名称**：*NXF1 is required for tyrosine kinase inhibitor-induced apoptosis in human EGFR-mutant lung cancer cells*
**作者**：Lee, H.J., et al.
**摘要**：研究通过Western blot和免疫组化使用NXF1抗体，发现EGFR突变肺癌细胞中NXF1表达下调会抑制靶向治疗诱导的细胞凋亡，提示NXF1在肺癌治疗耐药性中的潜在调控作用。
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3. **文献名称**：*Structural basis for the assembly of the nuclear pore complex*
**作者**：Fribourg, S., et al.
**摘要**：该文结合冷冻电镜和NXF1抗体介导的蛋白互作分析，解析了NXF1与核孔复合体(Nup)蛋白的结合模式，阐明了其在介导mRNA穿越核孔过程中的关键构象变化。
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**注**：以上文献信息为模拟示例，实际引用时请通过PubMed/Google Scholar检索具体论文并核对细节。
The NXF1 (Nuclear RNA Export Factor 1) antibody is a critical tool for studying the molecular mechanisms of mRNA export from the nucleus to the cytoplasm. NXF1. also known as TAP (Tip-associated protein), is a conserved eukaryotic protein that plays a central role in mediating the transport of mature mRNAs through nuclear pore complexes. It functions as part of a heterodimer with p15 (NXT1), binding to mRNA via adaptor proteins like Aly/REF and the exon junction complex. NXF1 contains distinct domains, including an RNA-binding domain (RBD) and leucine-rich repeats (LRM), enabling interactions with mRNA and nucleoporins.
Antibodies targeting NXF1 are widely used in research to investigate its expression, localization, and functional roles in RNA metabolism. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study NXF1's involvement in cellular processes, including viral replication (e.g., HIV-1. which hijacks NXF1 for viral RNA export) and cancer biology (e.g., aberrant NXF1 expression in malignancies). Additionally, NXF1 antibodies help elucidate its contributions to developmental defects and neurological disorders linked to mRNA export dysregulation. These antibodies are vital for both basic research and clinical studies aiming to understand NXF1's regulatory mechanisms and therapeutic potential.
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