| WB | 1/500-1/1000 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | NR4A1; GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3 |
| Entrez GeneID | 3164 |
| WB Predicted band size | Calculated MW: 64 kDa; Observed MW: 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | A synthetic peptide of human NUR77 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NUR77抗体的3篇参考文献示例(文献信息为虚拟模拟，仅供格式参考)：
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1. **文献名称**：*Nur77 regulates apoptosis in activated T cells through caspase-8 activation*
**作者**：Lin B. et al.
**摘要**：该研究利用NUR77抗体通过Western blot和免疫共沉淀技术，揭示了Nur77在活化T细胞中通过结合caspase-8诱导凋亡的分子机制，为自身免疫疾病治疗提供新靶点。
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2. **文献名称**：*Nur77 expression in hepatocellular carcinoma and its role in tumor suppression*
**作者**：Wu Y. et al.
**摘要**：作者通过免疫组化(使用NUR77特异性抗体)发现Nur77在肝癌组织中低表达，并通过体外实验证明其过表达抑制肿瘤细胞增殖，提示其作为肝癌预后标志物的潜力。
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3. **文献名称**：*Nur77 modulates hepatic lipid metabolism via AMPK signaling pathway*
**作者**：Pei L. et al.
**摘要**：研究采用NUR77抗体进行染色质免疫沉淀(ChIP)，证实Nur77直接调控脂代谢相关基因，并通过敲除小鼠模型揭示其在非酒精性脂肪肝中的保护作用。
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注：以上文献为示例性内容，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science搜索关键词“Nur77 antibody”或“NR4A1 antibody”获取具体文献。
The Nur77 antibody is a crucial tool for studying the Nur77 protein (also known as NR4A1), a member of the NR4A subfamily of nuclear receptors. Nur77 plays diverse roles in cellular processes, including apoptosis, inflammation, metabolism, and cancer progression. It functions as a transcription factor, regulating gene expression in response to stress signals, growth factors, or hormonal cues. Notably, Nur77 can translocate from the nucleus to mitochondria under specific conditions, triggering apoptosis through interactions with Bcl-2 family proteins.
Nur77 antibodies are designed to detect and quantify endogenous Nur77 protein levels in various experimental applications, such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies are typically raised against specific epitopes of human Nur77. with cross-reactivity tested in common model organisms like mice and rats. High-quality antibodies are validated for specificity using knockout controls or siRNA knockdown to ensure minimal off-target binding.
Research utilizing Nur77 antibodies has advanced understanding of its dual roles in promoting or suppressing tumors, depending on cellular context, as well as its involvement in metabolic diseases and autoimmune disorders. Its expression is often linked to T-cell receptor signaling, making it relevant in immune regulation studies. As a transiently induced early-response gene product, Nur77 detection requires careful experimental timing. Reliable antibodies are essential for exploring its dynamic subcellular localization and post-translational modifications, which dictate its functional outcomes.
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