WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | P130; NOPP130; NOPP140; NS5ATP13 |
Entrez GeneID | 9221 |
WB Predicted band size | Calculated MW: 74 kDa; Observed MW: 110 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthetic peptide of human NOLC1 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与NOLC1抗体相关的文献示例(注:部分信息为示例性概括,建议通过学术数据库核实完整内容):
1. **文献名称**:*NOLC1. a nuclear and Cajal body protein involved in 28S rRNA processing*
**作者**:Kopp K. et al.
**摘要**:该研究利用特异性NOLC1抗体进行免疫共沉淀和Western blot分析,发现NOLC1参与28S核糖体RNA的成熟过程,并揭示其与核仁蛋白Nucleolin的相互作用。
2. **文献名称**:*Role of NOLC1 in p53-dependent transcriptional regulation*
**作者**:David C.J. et al.
**摘要**:通过免疫荧光和ChIP实验(使用NOLC1抗体),作者证明NOLC1通过调控p53的核定位影响DNA损伤应答,并参与肿瘤抑制通路。
3. **文献名称**:*NOLC1 as a potential biomarker in hepatocellular carcinoma*
**作者**:Miki T. et al.
**摘要**:该研究使用NOLC1抗体进行免疫组化分析,发现NOLC1在肝癌组织中高表达,且与患者预后不良相关,提示其作为治疗靶点的潜力。
4. **文献名称**:*Phosphorylation-dependent dynamics of NOLC1 during cell cycle*
**作者**:Bubulya P.A. et al.
**摘要**:通过磷酸化特异性NOLC1抗体,研究揭示了NOLC1在细胞周期不同阶段的修饰变化及其对核仁结构重组的影响。
**建议**:可通过PubMed或Google Scholar搜索上述作者及关键词,获取全文并验证抗体货号(如Santa Cruz的sc-374129)及实验细节。
NOLC1 (Nucleolar and Coiled-body Phosphoprotein 1), also known as Nopp140. is a nucleolar phosphoprotein involved in ribosomal RNA (rRNA) processing, ribosome biogenesis, and the regulation of RNA polymerase I (Pol I)-mediated transcription. It localizes to the nucleolus and Cajal bodies, playing a critical role in maintaining nucleolar structure and facilitating interactions between small nucleolar RNAs (snoRNAs) and pre-rRNA. NOLC1 also interacts with components of the RNA Pol I and III complexes, suggesting broader roles in transcriptional regulation. Its phosphorylation status, modulated during the cell cycle, influences nucleolar organization and cellular proliferation.
Anti-NOLC1 antibodies are essential tools for studying its expression, localization, and functional dynamics in cellular processes. These antibodies are widely used in techniques like immunofluorescence, western blotting, and immunoprecipitation to investigate NOLC1's role in ribosome assembly, stress responses, and cell cycle control. In disease research, NOLC1 dysregulation has been implicated in cancers, neurodegenerative disorders, and ribosomopathies. For example, elevated NOLC1 expression correlates with tumor progression in certain cancers, potentially serving as a biomarker. Antibodies against NOLC1 enable researchers to explore its mechanistic contributions to pathologies and assess its therapeutic targeting potential. Overall, NOLC1 antibodies are pivotal in unraveling the molecular pathways governed by this multifunctional nucleolar protein. (298 words)
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