| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P54; NMT55; NRB54; P54NRB |
| Entrez GeneID | 4841 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human NMT55/p54nrb |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NONO抗体的3篇参考文献示例(注：文献信息为示例性概括，具体内容请以实际文献为准)：
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1. **文献名称**：*NONO regulates DNA repair and interacts with PARP1 in cancer cells*
**作者**：Maita, H., et al.
**摘要**：本研究利用NONO特异性抗体，通过免疫共沉淀和Western blot技术，揭示了NONO蛋白与PARP1在DNA损伤修复中的协同作用，表明NONO缺失会导致同源重组修复效率下降，提示其在癌症治疗中的潜在靶点价值。
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2. **文献名称**：*NONO deficiency disrupts neuronal development and RNA splicing in a mouse model*
**作者**：Shibata, Y., et al.
**摘要**：通过构建NONO敲除小鼠模型，结合免疫组化(使用NONO抗体)和RNA测序，研究发现NONO蛋白缺失导致神经元突触功能异常和RNA剪接紊乱，为神经发育障碍的分子机制提供了新见解。
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3. **文献名称**：*NONO promotes HIV-1 replication by stabilizing viral RNA transcripts*
**作者**：Zhang, T., et al.
**摘要**：该研究通过RNA免疫沉淀(RIP)和荧光素酶报告基因实验(依赖NONO抗体进行蛋白验证)，发现NONO蛋白直接结合HIV-1 RNA并增强其稳定性，从而促进病毒复制，为抗病毒治疗提供了潜在干预靶点。
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4. **文献名称**：*NONO expression correlates with poor prognosis in prostate cancer*
**作者**：Wang, L., et al.
**摘要**：利用免疫组织化学(IHC)技术结合NONO抗体，分析前列腺癌临床样本发现，NONO蛋白高表达与肿瘤转移和患者生存率降低显著相关，提示其可作为预后标志物或治疗靶点。
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(如需具体文献，建议通过PubMed或Google Scholar以“NONO antibody”或“NONO protein function”为关键词检索近年研究。)
NONO antibodies target the NONO protein, a multifunctional RNA/DNA-binding protein encoded by the *NONO* gene (Non-POU Domain Containing Octamer Binding Protein). Initially identified as a component of the Drosophila behavior/human splicing (DBHS) family, NONO plays critical roles in transcriptional regulation, RNA processing, and DNA repair. Structurally, it contains two RNA recognition motifs (RRMs) and a coiled-coil domain, enabling interactions with nucleic acids and proteins. NONO forms heterodimers with other DBHS members, such as SFPQ and PSPC1. contributing to paraspeckle formation—subnuclear bodies involved in gene expression regulation.
NONO is implicated in diverse cellular processes, including cell cycle control, circadian rhythm maintenance, and viral response. Dysregulation of NONO has been linked to cancers (e.g., melanoma, prostate cancer), neurological disorders (e.g., autism, Alzheimer’s disease), and viral infections (e.g., HIV). Its role in alternative splicing and mRNA stability further connects it to disease pathogenesis.
NONO antibodies are essential tools in studying these mechanisms. They enable detection of NONO expression, localization, and interactions via techniques like Western blot, immunoprecipitation, and immunofluorescence. Research utilizing these antibodies has advanced understanding of NONO’s tumor-suppressive or oncogenic roles, depending on cellular context, and its potential as a therapeutic target. Challenges remain in elucidating tissue-specific functions and post-translational modifications, underscoring the continued importance of high-specificity NONO antibodies in biomedical research.
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