首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人黄嘌呤氧化酶(XOD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的XDH含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，为研究其在痛风、心血管疾病、糖尿病等病理机制中的作用提供可靠工具；科研人员可将其用于动物模型或体外实验中XOD活性调控研究、药物筛选评估，或结合多组学数据探索代谢相关疾病的生物标志物，为生物医学研究提供高效的分析解决方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Xanthine dehydrogenase ELISA Kit; Xanthine dehydrogenase/oxidase ELISA Kit; Xanthine oxidase ELISA Kit; Xanthine oxidoreductase ELISA Kit; XD ELISA Kit; XDH ELISA Kit; XDH_HUMAN ELISA Kit; xdha ELISA Kit; XO ELISA Kit; xor ELISA Kit
缩写：XDH
Uniprot No.：P47989
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human XOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of human XOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 92-107 | ||||
| EDTA plasma (n=4) | 99 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 101.8631.7991.8311.710 51.4001.4331.4171.296 2.50.8950.9190.9070.786 1.250.5400.5270.5340.413 0.6250.3470.3410.3440.223 0.3120.2330.2390.2360.115 0.1560.1730.1840.1790.058 00.1200.1220.121 | ||||||
货期：3-5 working days
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