| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BSC1; NKCC2 |
| Entrez GeneID | 6557 |
| WB Predicted band size | Calculated MW: 121 kDa; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human NKCC2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SLC12A1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Molecular cloning and characterization of the renal Na-K-Cl cotransporter (NKCC2/SLC12A1)"*
**作者**：Gamba, G., et al.
**摘要**：该研究首次克隆了SLC12A1基因编码的NKCC2蛋白，并生成特异性抗体。通过Western blot和免疫组化验证了NKCC2在肾脏髓袢升支粗段(TAL)的特异性表达，为后续研究其在盐重吸收中的作用奠定基础。
2. **文献名称**：*"A monoclonal antibody targeting the Na-K-Cl cotransporter (NKCC2/SLC12A1) reveals its apical localization in renal epithelial cells"*
**作者**：Gimenez, I., Forbush, B.
**摘要**：研究团队开发了一种针对NKCC2的单克隆抗体，并通过免疫荧光和电镜证实其位于肾小管上皮细胞的顶膜侧。抗体特异性验证显示其适用于检测不同物种(如大鼠、小鼠)的NKCC2蛋白表达。
3. **文献名称**：*"Regulation of the renal Na+-K+-2Cl− cotransporter (NKCC2/SLC12A1) by dietary potassium"*
**作者**：Castrop, H., et al.
**摘要**：利用SLC12A1抗体研究低钾饮食对NKCC2表达的影响。Western blot和免疫组化显示，低钾条件下NKCC2蛋白在TAL的表达上调，提示其在钾稳态调节中的关键作用。
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以上文献均涉及SLC12A1抗体的开发或应用，涵盖基因功能验证、蛋白定位及病理生理机制研究。
The SLC12A1 antibody targets the solute carrier family 12 member 1 (SLC12A1) protein, also known as the Na-K-Cl cotransporter 2 (NKCC2). This transmembrane protein is predominantly expressed in the thick ascending limb of the loop of Henle in the kidney, where it plays a critical role in electrolyte homeostasis by mediating the reabsorption of sodium, potassium, and chloride ions from the renal filtrate. NKCC2 is essential for maintaining body fluid balance, blood pressure regulation, and urinary concentration. Dysfunction of SLC12A1 is linked to autosomal recessive Bartter syndrome type 1. a life-threatening salt-wasting disorder characterized by polyuria, hypokalemia, and metabolic alkalosis.
SLC12A1 antibodies are widely used in research to study the expression, localization, and functional role of NKCC2 in physiological and pathological contexts. These antibodies are typically developed in hosts such as rabbits or mice, using immunogenic peptides derived from conserved regions of the protein. They enable detection of NKCC2 via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Validation often includes testing in knockout models or tissues to confirm specificity. Research applications focus on renal physiology, hypertension, electrolyte disorders, and drug development targeting ion transport mechanisms. The antibody’s utility extends to elucidating molecular mechanisms underlying Bartter syndrome and exploring therapeutic interventions for related tubular pathologies.
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