首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述:小鼠黄嘌呤脱氢酶/氧化酶(XDH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的XDH含量。XDH即黄嘌呤脱氢酶,在嘌呤代谢中发挥关键作用,能催化黄嘌呤生成尿酸。其背景在于与痛风等因尿酸代谢异常引发的疾病密切相关。研究机制主要聚焦于寻找能抑制该靶点活性的物质,以减少尿酸生成,为相关疾病治疗提供新策略。试剂盒检测范围为7.8 pg/mL-500 pg/mL,产品适用于基础科研领域,如探究肝脏疾病、高尿酸血症、代谢综合征等动物模型中XDH的生物学功能,或用于评估氧化应激相关信号通路的分子机制研究。支持实验室常规开展小鼠模型的分子水平检测,为代谢调控、病理机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Xdh ELISA Kit; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase ELISA Kit; XD ELISA Kit; EC 1.17.1.4); Xanthine oxidase ELISA Kit; XO ELISA Kit; EC 1.17.3.2 ELISA Kit; Xanthine oxidoreductase ELISA Kit; XOR)] ELISA Kit
缩写:XDH
Uniprot No.:Q00519
种属:Mus musculus (Mouse)
样本类型:serum, plasma, tissue homogenates
检测范围:7.8 pg/mL-500 pg/mL
灵敏度:1.95 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse XDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1000 | Average % | 91 | |||||
Range % | 87-95 | ||||||
1:2000 | Average % | 98 | |||||
Range % | 94-102 | ||||||
1:4000 | Average % | 92 | |||||
Range % | 87-98 | ||||||
1:8000 | Average % | 92 | |||||
Range % | 88-97 |
回收率:
The recovery of mouse XDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 101 | 97-104 | |||||
EDTA plasma (n=4) | 104 | 96-108 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 5002.6542.5162.5852.492 2501.8241.7141.7691.676 1251.1651.1261.1461.053 62.50.6430.6530.6480.555 31.20.3870.3650.3760.283 15.60.2240.2390.2320.139 7.80.1590.1550.1570.064 00.0940.0920.093 |
货期:3-5 working days
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