首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Wilms肿瘤蛋白(WT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WT1含量。WT1即Wilms瘤基因1，是一种重要的肿瘤相关靶点。它在胚胎发育中发挥关键作用，在多种恶性肿瘤中异常高表达。研究表明其可通过影响细胞增殖、凋亡、分化等机制参与肿瘤发生发展，是颇具潜力的肿瘤治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可为肿瘤微环境研究、抗癌药物作用机制解析以及疾病相关蛋白表达动态监测提供可靠工具，特别适用于体外培养细胞模型中WT1调控机制的探索及临床前动物实验的疗效评估，其宽泛的样本兼容性可满足基础科研中多维度实验设计需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：WIT 2 ELISA Kit; WT 1 ELISA Kit; AWT1 ELISA Kit; FWT1 ELISA Kit; GUD ELISA Kit; NPHS4 ELISA Kit; WAGR ELISA Kit; Wilms tumor 1 ELISA Kit; Wilms Tumor ELISA Kit; Wilms tumor protein ELISA Kit; Wilms' tumor gene ELISA Kit; Wilms' tumor protein ELISA Kit; WIT2 ELISA Kit; WT ELISA Kit; WT1 ELISA Kit; WT1_HUMAN ELISA Kit; WT33 ELISA Kit
缩写：WT1
Uniprot No.：P19544
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 95-101 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 91-97 | |||||
回收率：
| The recovery of human WT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 99 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4232.4942.4592.3305001.9311.9051.9181.7892501.4961.5791.5381.4091251.1651.1941.1801.05162.50.8220.8450.8340.70531.20.4750.4960.4860.35715.60.2600.2550.2580.12900.1240.1330.129 | ||||||
货期：3-5 working days
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