首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Wnt-7b蛋白(WNT7B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT7B含量。WNT7B 是一种重要的靶点。它属于 WNT 蛋白家族成员，在细胞生长、分化、组织稳态维持等多种生理过程中起关键作用。研究机制主要围绕其参与 WNT 信号通路，异常激活或抑制该通路与多种疾病如肿瘤的发生发展密切相关。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可广泛应用于发育生物学、疾病机制研究及药物开发等科研领域，例如探究Wnt - 7b在特定组织中的表达调控、评估基因编辑或药物干预对信号通路的影响等。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt7b ELISA Kit; Wnt-7b ELISA Kit; Protein Wnt-7b ELISA Kit
缩写：WNT7B
Uniprot No.：P28047
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT7B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-105 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-101 | |||||
回收率：
| The recovery of mouse WNT7B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5802.6182.5992.447101.5321.4821.5071.35550.9330.8950.9140.7622.50.5790.5860.5830.4311.250.3520.3460.3490.1970.6250.2580.2690.2640.1120.3120.2070.2130.2100.05800.1550.1490.152 | ||||||
货期：3-5 working days
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