| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CTF; HMGIC/NFIB; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; Nfib; NFIB2; NFIB3 |
| Entrez GeneID | 4781 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47,55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human NFIB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NFIB抗体的3篇文献摘要及作者信息：
1. **"NFIB promotes cell proliferation by regulating the ATM signaling pathway in colorectal cancer"**
- **作者**: Li, X., et al.
- **摘要**: 研究通过NFIB抗体检测发现，NFIB在结直肠癌中高表达，并通过调控ATM信号通路促进肿瘤细胞增殖，提示其作为潜在治疗靶点的可能性。
2. **"Nuclear Factor I/B (NFIB) regulates metastatic potential in melanoma"**
- **作者**: Smith, J.R., et al.
- **摘要**: 利用NFIB抗体进行免疫组化分析，发现NFIB在黑色素瘤转移中通过调控基质重塑相关基因表达驱动侵袭性表型，为转移机制提供新见解。
3. **"NFIB deficiency inhibits lung maturation through transcriptional dysregulation"**
- **作者**: Chang, A.N., et al.
- **摘要**: 通过NFIB抗体在小鼠模型中的染色实验，揭示NFIB缺失导致肺发育不全，其通过调控肺泡上皮细胞分化相关基因影响器官成熟。
(注：上述文献信息为示例性质，实际引用需根据具体论文核实。)
**Background of NFIB Antibodies**
NFIB (Nuclear Factor I B) antibodies are immunological tools used to detect and study the NFIB protein, a member of the Nuclear Factor I (NFI) family of transcription factors. NFIB plays critical roles in regulating gene expression by binding to specific DNA sequences, influencing cellular processes such as proliferation, differentiation, and development. Structurally, NFIB contains an N-terminal DNA-binding domain and a C-terminal transactivation/transrepression domain, enabling it to modulate transcriptional activity.
NFIB is implicated in various biological and pathological contexts. During embryogenesis, it is essential for organ development, particularly in the brain, lung, and salivary glands. Aberrant NFIB expression or function has been linked to cancers, including small-cell lung carcinoma, glioblastoma, and breast cancer, where it may act as a proto-oncogene by promoting cell survival, migration, or metastasis.
NFIB antibodies are widely used in research to investigate protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). These antibodies help identify NFIB isoforms generated by alternative splicing, which may exhibit distinct functional roles.
Recent studies also explore NFIB's involvement in epigenetic regulation and its potential as a diagnostic or prognostic biomarker. However, challenges remain in understanding isoform-specific functions and context-dependent roles. NFIB antibodies thus serve as vital reagents for unraveling the protein's contributions to both normal physiology and disease.
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