Human Protein Wnt-7b(WNT7B) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL026142HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人Wnt-7b蛋白(WNT7B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的WNT7B含量。WNT7B 是重要靶点。它参与 Wnt 信号通路，在胚胎发育、细胞增殖与分化等方面发挥关键作用。研究其机制有助于揭示疾病发生发展原理，为相关疾病如癌症等的诊断、治疗及药物研发提供新方向与潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础研究领域，如探究Wnt信号通路在组织修复、干细胞分化或疾病模型中的作用机制，尤其适合需要从复杂生物样本（如炎症相关组织匀浆或循环系统样本）中定量分析WNT7B表达变化的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Protein Wnt-7b ELISA Kit; Wingless related MMTV integration site 7B ELISA Kit; Wingless type MMTV integration site family member 7B ELISA Kit; WNT ELISA Kit; WNT7B ELISA Kit; WNT7B_HUMAN ELISA Kit
缩写：
WNT7B
Uniprot No.：
P56706
种属：
Homo sapiens (Human)
样本类型：
serum, plasma,tissue homogenates
检测范围：
0.156 ng/mL-10 ng/mL
灵敏度：
0.039 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Stem Cells
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human WNT7B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	102
	Range %	95-108
1:2	Average %	96
	Range %	91-100
1:4	Average %	87
	Range %	82-93
1:8	Average %	95
	Range %	88-102

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.532	2.634	2.583	2.444
5	1.683	1.767	1.725	1.586
2.5	0.901	0.928	0.915	0.775
1.25	0.554	0.579	0.567	0.427
0.625	0.363	0.349	0.356	0.217
0.312	0.272	0.259	0.265	0.126
0.156	0.189	0.194	0.191	0.052
0	0.143	0.136	0.139