| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | neurogranin (protein kinase C substrate; RC3); RC3; hng |
| Entrez GeneID | 4900 |
| WB Predicted band size | Calculated MW: 8 kDa; Observed MW: 14-16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Neurogranin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Neurogranin抗体的3篇参考文献的简要信息：
1. **文献名称**: *Neurogranin as a cognitive biomarker in cerebrospinal fluid and blood*
**作者**: Kester, M.I., et al.
**摘要**: 该研究探讨了脑脊液和血液中Neurogranin作为阿尔茨海默病(AD)生物标志物的潜力，发现其水平与认知功能衰退相关，并验证了特异性抗体在检测中的可靠性。
2. **文献名称**: *Characterization of novel antibodies against Neurogranin for postmortem studies of human brain*
**作者**: Portelius, E., et al.
**摘要**: 研究团队开发并验证了针对Neurogranin的新型抗体，证实其在人脑组织切片中的特异性染色能力，为突触可塑性和AD病理研究提供了工具。
3. **文献名称**: *Assessment of pre-analytical and analytical variables in Neurogranin measurement*
**作者**: Kvartsberg, H., et al.
**摘要**: 通过比较不同Neurogranin抗体的灵敏度和特异性，该文献提出了优化脑脊液样本检测的标准化流程，强调了抗体选择对实验结果的影响。
4. **文献名称**: *Synaptic protein Neurogranin is validated as a biomarker for Alzheimer’s disease in multiple cohorts*
**作者**: Anton Alvarez, A., et al.
**摘要**: 利用多种抗体平台验证了Neurogranin在AD患者脑脊液中的升高现象，支持其作为突触损伤标志物的临床价值。
以上文献均聚焦于Neurogranin抗体的开发、验证及其在神经疾病诊断中的应用。
Neurogranin (Ng), a small postsynaptic protein enriched in the brain, plays a critical role in synaptic plasticity, learning, and memory. It functions as a calmodulin (CaM)-binding protein, regulating calcium signaling by sequestering CaM in dendritic spines under low calcium conditions. Upon calcium influx, CaM dissociates from Ng to activate downstream targets like CaMKII, facilitating long-term potentiation (LTP). Structurally, Ng contains an IQ domain essential for CaM interaction and multiple phosphorylation sites modulating its activity.
Neurogranin antibodies are vital tools for studying its expression, localization, and function in neurological disorders. Reduced Ng levels in cerebrospinal fluid (CSF) and brain tissue correlate with synaptic loss in Alzheimer’s disease (AD), making Ng a potential biomarker for early neurodegeneration. Antibodies targeting Ng enable its detection via techniques like Western blot, immunohistochemistry, and ELISA, aiding research on synaptic integrity and disease mechanisms. Recent studies highlight CSF Ng as a predictor of cognitive decline in AD, driving interest in standardized assays for clinical use. However, challenges remain in optimizing antibody specificity across isoforms and post-translationally modified forms. Ongoing research focuses on validating Ng antibodies for diagnostic applications and exploring therapeutic strategies targeting Ng-mediated pathways to preserve synaptic health.
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