首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Wnt-3a蛋白(WNT3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT3A含量。WNT3A 是一种重要的信号蛋白。它在胚胎发育、细胞增殖与分化等过程中发挥关键作用。其研究机制主要围绕 WNT 信号通路，异常激活或抑制该通路会引发发育异常及疾病，如肿瘤，对它的深入研究有助于疾病治疗手段的探索。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于科研领域研究Wnt信号通路的激活状态、干细胞定向分化调控、肿瘤微环境分析或发育生物学模型构建等实验场景，支持多种生物样本类型检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt3a ELISA kit; Wnt-3aProtein Wnt-3a ELISA kit
缩写：WNT3A
Uniprot No.：P27467
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 95-100 | |||||
回收率：
| The recovery of mouse WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-95 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.8862.8642.8752.7787502.0212.0112.0161.9193751.1521.1321.1421.045187.50.6450.6380.6420.545940.3640.3780.3710.274470.2390.2550.2470.15023.50.1620.1720.1670.07000.0950.0980.097 | ||||||
货期：3-5 working days
×
