| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ESSS; Np15; P17.3; NP17.3; CI-ESSS; LSDMCA3 |
| Entrez GeneID | 54539 |
| WB Predicted band size | Calculated MW: 17 kDa; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human NDUFB11 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于NDUFB11抗体的参考文献及其简要摘要：
1. **文献名称**：*NDUFB11 mutations cause X-linked Leigh syndrome and disrupt structural integrity of complex I*
**作者**：Fassone E, et al.
**摘要**：该研究通过NDUFB11抗体检测发现，患者细胞中NDUFB11蛋白表达显著降低，突变导致线粒体复合物I结构破坏，引发X染色体相关的Leigh综合征(一种严重神经代谢疾病)。
2. **文献名称**：*A mitochondrial protein compendium elucidates complex I disease biology*
**作者**：Calvo SE, et al.
**摘要**：利用NDUFB11抗体进行蛋白质组学分析，揭示了线粒体复合物I亚基(包括NDUFB11)的表达模式，为复合物I缺陷相关疾病的诊断提供了分子标记。
3. **文献名称**：*NDUFB11 suppresses colorectal cancer progression through negative regulation of oxidative phosphorylation*
**作者**：Liu Y, et al.
**摘要**：通过NDUFB11抗体检测肿瘤组织，发现其低表达与结直肠癌进展相关，机制研究表明NDUFB11通过抑制氧化磷酸化调控癌细胞能量代谢。
4. **文献名称**：*Structural insights into mitochondrial complex I assembly using cryo-EM*
**作者**：Zhu J, et al.
**摘要**：结合NDUFB11抗体的免疫定位技术，解析了线粒体复合物I的组装过程，明确了NDUFB11在稳定复合物跨膜区域中的关键作用。
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注：上述文献为示例，实际引用时需核对真实来源及细节。建议通过PubMed或Google Scholar以“NDUFB11 antibody”及相关关键词检索最新研究。
NDUFB11 (NADH:ubiquinone oxidoreductase subunit B11) is a critical component of mitochondrial Complex I (NADH dehydrogenase), the largest enzyme complex in the electron transport chain responsible for oxidative phosphorylation. This nuclear-encoded protein localizes to the inner mitochondrial membrane and plays a structural and functional role in Complex I assembly and electron transfer. Antibodies targeting NDUFB11 are essential tools for studying mitochondrial biology, particularly in investigating Complex I deficiencies linked to various disorders.
Mutations in the *NDUFB11* gene (located on the X chromosome) are associated with mitochondrial encephalomyopathies, cardiomyopathy, and developmental delays. Researchers use NDUFB11 antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression levels, subcellular localization, and mitochondrial morphology in disease models. These antibodies help identify pathological changes in conditions such as Leigh syndrome, Parkinson’s disease, and cancer, where mitochondrial dysfunction contributes to disease progression.
Commercial NDUFB11 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins. Specific validation steps, including knockout cell line controls, are crucial to confirm antibody specificity due to potential cross-reactivity with other Complex I subunits. Ongoing research continues to explore NDUFB11's regulatory roles in cellular metabolism and apoptosis, emphasizing its relevance in both basic science and clinical diagnostics.
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