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产品描述：人Wnt-16蛋白(WNT16)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT16含量。WNT16是一个重要靶点。它属于WNT信号通路成员，在胚胎发育、组织稳态维持等过程起关键作用。研究主要聚焦其在肿瘤发生发展中的机制，揭示它如何调控细胞增殖、迁移及肿瘤微环境等，有望为肿瘤诊断和治疗提供新方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于基础研究领域，如骨代谢相关信号通路机制探索、肿瘤微环境与转移关系研究、骨骼发育异常模型分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Protein Wnt 16 precursor ELISA Kit; Protein Wnt-16 ELISA Kit; Wingless type MMTV integration site family member 16 ELISA Kit; WNT16 ELISA Kit; WNT16_HUMAN ELISA Kit
缩写：WNT16
Uniprot No.：Q9UBV4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of human WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-92 | ||||
| EDTA plasma (n=4) | 100 | 97-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4522.4872.4702.29310001.7161.7361.7261.5495001.1111.1491.1300.9532500.6730.6980.6860.5091250.4650.4680.4670.29062.50.3410.3620.3520.17531.250.2870.2760.2820.10500.1760.1780.177 | ||||||
货期：3-5 working days
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