首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Wnt-10b蛋白(WNT10B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT10B含量。WNT10B是Wnt蛋白家族成员，参与细胞命运调控和肿瘤转化。研究显示，WNT10B在脂肪、骨骼、肝、胃肠和皮肤中调控细胞多能性，与乳腺癌、肿瘤性转化等疾病相关。其研究机制涉及Wnt信号通路调控细胞增殖、分化等过程。试剂盒检测范围为9.4 pg/mL-600 pg/mL，可应用于发育生物学、肿瘤机制研究、代谢性疾病模型构建及骨骼发育相关实验，为探索WNT10B在细胞信号传导、组织再生等领域的调控机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt10b ELISA Kit; Wnt-10b ELISA Kit; Wnt10 ELISA Kit; Wnt12 ELISA Kit; Protein Wnt-10b ELISA Kit; Protein Wnt-12 ELISA Kit
缩写：WNT10B
Uniprot No.：P48614
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：9.4 pg/mL-600 pg/mL
灵敏度：2.35 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT10B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 97-106 | |||||
回收率：
| The recovery of mouse WNT10B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 84-90 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 6002.0482.1542.1011.943 3001.3881.3981.3931.235 1500.7450.7140.7300.572 750.4040.4350.4200.262 37.50.3000.3210.3110.153 18.750.2640.2780.2710.113 9.380.2050.2090.2070.049 00.1590.1570.158 | ||||||
货期：3-5 working days
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