| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHRM2; HM2; AChR; Acm2 |
| Entrez GeneID | 1129 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human Muscarinic Acetylcholine Receptor 2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3-4篇关于Muscarinic Acetylcholine Receptor M2(M2受体)抗体的代表性文献，按文献名称、作者和摘要内容概括：
---
1. **文献名称**: "Selective activation of intracellular signaling pathways in cardiomyocytes by M2 muscarinic acetylcholine receptor-specific antibodies"
**作者**: Bunemann, M. et al.
**摘要**: 研究利用M2受体特异性抗体，揭示其在心肌细胞中选择性激活G蛋白偶联信号通路的机制，表明M2受体抗体可用于探究心脏胆碱能调控的分子基础。
2. **文献名称**: "Autoantibodies against the β1-adrenergic and M2 muscarinic receptors in transgenic mouse models of heart failure"
**作者**: Wang, W. et al.
**摘要**: 通过转基因小鼠模型，发现针对M2受体的自身抗体可诱导心肌功能异常，提示其在心力衰竭病理中的潜在作用，为抗体在疾病模型中的应用提供依据。
3. **文献名称**: "Anti-M2 muscarinic receptor autoantibodies in patients with dilated cardiomyopathy"
**作者**: Fu, L.X. et al.
**摘要**: 临床研究发现扩张型心肌病患者血清中存在M2受体自身抗体，其与心肌收缩功能抑制相关，支持抗体作为疾病诊断和机制研究的生物标志物。
4. **文献名称**: "Development of a monoclonal antibody targeting the extracellular domain of M2 muscarinic receptor for functional studies"
**作者**: Hosey, M.M. et al.
**摘要**: 报道一种靶向M2受体胞外域的单克隆抗体的开发，验证其特异性阻断乙酰胆碱结合的能力，为受体结构和药物开发研究提供工具。
---
这些文献涵盖了M2受体抗体在基础机制研究、疾病模型构建、临床诊断及药物开发中的关键应用。
The muscarinic acetylcholine receptor M2 (M2 mAChR) is a G protein-coupled receptor (GPCR) predominantly found in the heart, smooth muscles, and specific regions of the central and peripheral nervous systems. As one of five muscarinic receptor subtypes (M1-M5), M2 binds acetylcholine to mediate parasympathetic nervous system responses, including heart rate reduction, decreased cardiac contractility, and smooth muscle relaxation. Structurally, M2 couples to inhibitory Gi/o proteins, suppressing adenylyl cyclase activity and reducing intracellular cAMP levels.
Antibodies targeting M2 mAChR are primarily studied in research and clinical contexts. Autoantibodies against M2 have been implicated in autoimmune and cardiovascular disorders, such as dilated cardiomyopathy, Chagas disease, and Sjögren’s syndrome, where they may disrupt normal receptor signaling, leading to arrhythmias or autonomic dysfunction. In research, M2-specific antibodies are tools for detecting receptor expression, mapping tissue distribution, or modulating receptor activity in experimental models. Clinically, their detection aids in diagnosing autoimmune conditions or understanding drug interactions affecting M2 function.
Therapeutic applications are emerging, with M2-targeting antibodies explored for their potential to regulate pathological receptor overactivity or restore autonomic balance. However, their role remains complex due to the receptor’s dual involvement in excitatory and inhibitory signaling across diverse tissues.
×
