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产品描述：小鼠WAP四-二硫核心结构域蛋白2(WFDC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WFDC2含量。小鼠WAP四-二硫核心结构域蛋白2(WFDC2)是一种分泌蛋白，含有两个WAP结构域，主要在男性生殖系统、呼吸道和鼻咽部表达。其在多种肿瘤细胞系中高表达，可能参与肿瘤的发生发展，并与支气管扩张有关，其研究机制涉及基因变异和蛋白质功能。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础科研中探索WFDC2在疾病模型（如炎症性疾病、纤维化病变或代谢紊乱）中的生物学功能，分析其在组织损伤修复中的动态变化，或评估特定干预措施对WFDC2表达水平的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wfdc2 ELISA Kit; He4 ELISA Kit; WAP four-disulfide core domain protein 2 ELISA Kit; WAP domain-containing protein HE4 ELISA Kit
缩写：WFDC2
Uniprot No.：Q9DAU7
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WFDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of mouse WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 92 | 86-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.0452.1442.0951.943 20001.6241.7431.6841.532 10001.0531.0721.0630.911 5000.6730.6630.6680.516 2500.3420.3510.3470.195 1250.2740.2830.2790.127 62.50.2290.2350.2320.080 00.1520.1510.152 | ||||||
货期：3-5 working days
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