| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A; MRE11A; HNGS1; MRE11 |
| Entrez GeneID | 4361 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Mre11 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇关于Mre11抗体的参考文献，按文献名称、作者和摘要内容概括整理：
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1. **"Mre11 Structure-Specific Endonuclease: Functional Roles in DNA Repair and Checkpoint Signaling"**
*作者：R.S. Williams et al. (2007)*
**摘要**：研究通过X射线晶体学解析Mre11蛋白的结构，并利用特异性抗体验证其核酸酶活性在DNA双链断裂修复中的作用。抗体用于免疫沉淀实验，证实Mre11与Rad50的结合模式。
2. **"The Mre11 Complex: At the Crossroads of DNA Repair and Checkpoint Signalling"**
*作者：T. Stracker et al. (2004)*
**摘要**：综述Mre11复合体(Mre11/Rad50/Nbs1)在DNA损伤应答中的核心功能。文中提到使用Mre11抗体进行免疫荧光染色，揭示其在电离辐射后病灶定位的动态变化。
3. **"Functional Analysis of the Mre11-Rad50-Nbs1 Complex in DNA Damage Responses"**
*作者：T. Uziel et al. (2003)*
**摘要**：通过敲除实验结合Mre11抗体检测蛋白表达水平，证明该复合体对ATM激酶激活的必要性。抗体用于Western blot和共聚焦显微镜观察细胞核内修复焦点形成。
4. **"Mre11 Dimers Coordinate DNA End Bridging and Nuclease Processing in Double-Strand Break Repair"**
*作者：K. Lobachev et al. (2002)*
**摘要**：利用抗Mre11抗体的免疫共沉淀技术，揭示Mre11二聚体在DNA末端桥接和加工中的双重作用，支持同源重组修复机制模型。
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以上文献均涉及Mre11抗体的实验应用(如Western blot、免疫沉淀、免疫荧光等)，并围绕其功能、结构或调控机制展开研究。
The Mre11 antibody is a crucial tool in studying the Mre11 protein, a key component of the MRN complex (Mre11-Rad50-NBS1), which plays a central role in DNA damage response and repair. Mre11. a conserved nuclease, is essential for processing double-strand breaks (DSBs) during homologous recombination (HR) and non-homologous end joining (NHEJ). It facilitates DNA end resection, checkpoint activation, and telomere maintenance. Dysregulation of Mre11 is linked to genomic instability, cancer predisposition, and neurodegenerative disorders.
Antibodies targeting Mre11 are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help elucidate Mre11's role in DNA repair pathways, cell cycle regulation, and responses to ionizing radiation or chemotherapeutic agents. Specific Mre11 antibodies are validated for cross-reactivity in various species (e.g., human, mouse) and are critical for assessing MRN complex integrity in diseases such as ataxia-telangiectasia-like disorder (ATLD) or cancers with defective DNA repair.
Researchers also use these antibodies to explore Mre11’s structural dynamics, post-translational modifications (e.g., phosphorylation), and its interplay with other repair proteins like BRCA1/2. High-quality, specificity-verified antibodies are vital to avoid false signals, given homologous domains in related proteins. Commercial Mre11 antibodies often cite validation in knockout cell lines or functional assays, ensuring reliability in both basic and translational studies.
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