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产品描述：人色氨酰tRNA合成酶(WARS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的WARS含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研领域中对细胞应激机制、信号通路调控或疾病模型（如炎症、肿瘤等）中WARS蛋白表达水平变化的定量研究，为探索该酶在细胞生物学过程中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gamma 2 ELISA Kit; GAMMA-2 ELISA Kit; Gamma2 ELISA Kit; hWRS ELISA Kit; IFI 53 ELISA Kit; IFI53 ELISA Kit; IFP 53 ELISA Kit; IFP53 ELISA Kit; Interferon induced protein 53 ELISA Kit; Interferon-induced protein 53 ELISA Kit; SYWC_HUMAN ELISA Kit; T2-TrpRS ELISA Kit; TrpRS ELISA Kit; Tryptophan tRNA ligase; cytoplasmic ELISA Kit; Tryptophan tRNA ligase 1 cytoplasmic ELISA Kit; Tryptophan tRNA ligase ELISA Kit; Tryptophan--tRNA ligase ELISA Kit; Tryptophanyl tRNA synthetase ELISA Kit; Tryptophanyl tRNA synthetase cytoplasmic ELISA Kit; WARS ELISA Kit; WARS protein ELISA Kit; WRS ELISA Kit
缩写：WARS
Uniprot No.：P23381
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 81-93 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 94-106 | |||||
回收率：
| The recovery of human WARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-95 | ||||
| EDTA plasma (n=4) | 93 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 201.9722.0502.0111.920 101.4461.5561.5011.410 51.0271.1011.0640.973 2.50.6380.6060.6220.531 1.250.3810.3870.3840.293 0.6250.2930.2750.2840.193 0.3120.1950.2010.1980.107 00.0930.0890.091 | ||||||
货期：3-5 working days
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