首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VWF含量。VWF即血管性血友病因子，在止血过程中发挥重要作用。其背景上与血管性血友病等疾病密切相关。研究机制方面，它能介导血小板黏附于受损血管壁，还可与凝血因子Ⅷ结合，保护其不被快速降解，目前围绕它的研究有助于疾病诊断与治疗。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于心血管疾病模型构建、血栓机制研究、药物疗效评估等科研场景。为探索VWF在生理病理过程中的作用提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
缩写：VWF
Uniprot No.：Q62935
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-99 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.3002.4882.3942.257 102.0112.1022.0571.920 51.4461.5601.5031.366 2.50.9030.9000.9020.765 1.250.6280.5860.6070.470 0.6250.3500.3640.3570.220 0.3120.2420.2580.2500.113 00.1340.1400.137 | ||||||
货期：3-5 working days
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