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Pig von Willebrand Factor,vWF ELISA Kit

  • 中文名: 猪血管性血友病因子/瑞斯托霉素辅因子(VWF)ELISA Kit
货号: IPD-E09395p
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    猪血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的VWF含量。VWF(血管性血友病因子)是一种大分子糖蛋白。在生理状态下,它参与血小板黏附、聚集以维持正常止血功能。病理方面,其水平异常与多种疾病如血管性血友病等相关。研究常聚焦于其结构功能关系、与疾病相互作用机制,为相关疾病诊断治疗提供依据。试剂盒检测范围为0.625 ng/mL-40 ng/mL,该产品可应用于猪凝血机制研究、出血性疾病动物模型建立、兽用止血药物开发评估,或畜牧养殖中猪群凝血功能的群体健康监测等科研场景,为探究VWF在生理病理过程中的调控机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    VWF ELISA kit; F8VWFvon Willebrand factor ELISA kit; vWF ELISA kit; Fragment ELISA kit
  • 缩写:
    VWF
  • Uniprot No.:
    Q28833
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma
  • 检测范围:
    0.625 ng/mL-40 ng/mL
  • 灵敏度:
    0.156 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of pig vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    93


    Range %

    89-99


    1:200

    Average %

    98


    Range %

    92-104


    1:400

    Average %

    89


    Range %

    82-95


    1:800

    Average %

    88


    Range %

    82-94


  • 回收率:

    The recovery of pig vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    91-103


    EDTA plasma (n=4)

    88

    84-94


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average



    40

    0.084

    0.085

    0.085



    20

    0.177

    0.174

    0.176



    10

    0.365

    0.375

    0.370



    5

    0.692

    0.704

    0.698



    2.5

    1.253

    1.244

    1.249



    1.25

    1.701

    1.714

    1.708



    0.625

    2.023

    2.121

    2.072



    0

    2.323

    2.425

    2.374




  • 货期:
    3-5 working days

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