首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管性血友病因子/瑞斯托霉素辅因子(vWF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VWF含量。VWF即血管性血友病因子，在止血过程中起关键作用，其缺陷会引发血管性血友病。研究机制方面，它可与血小板糖蛋白及内皮下胶原结合，促进血小板黏附聚集；还能作为载体蛋白结合Ⅷ因子，防止其被降解，在凝血途径中发挥重要功能。试剂盒检测范围为0.78 ng/mL-50 ng/mL，为研究小鼠模型中的血管功能调控、出血性疾病机理、抗血栓药物作用效果等提供可靠数据支持，尤其适用于心血管疾病动物模型、基因编辑小鼠的表型分析以及凝血相关生物标志物的基础研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vwfvon Willebrand factor ELISA kit; vWF) [Cleaved into: von Willebrand antigen 2 ELISA kit; von Willebrand antigen II)] ELISA kit
缩写：VWF
Uniprot No.：Q8CIZ8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of mouse vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.3232.4512.3872.293251.6451.7881.7171.62312.51.0671.0211.0440.9506.250.6020.5920.5970.5033.120.2830.3020.2930.1991.560.2450.2350.2400.1460.780.1660.1690.1680.07400.0920.0960.094 | ||||||
货期：3-5 working days
×
