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产品描述：犬血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VWF含量。VWF即血管性血友病因子，其在止血过程中发挥关键作用。当血管受损时，VWF能介导血小板黏附于受损血管壁。研究机制上多围绕其结构与功能关系，以及与其他凝血因子相互作用，目前它也是出血性和血栓性疾病研究的重要靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于科研场景中犬类VWF蛋白表达水平的定量分析，可用于探究凝血相关疾病的分子机制、评估实验动物模型的血栓或出血性病理状态，以及筛选影响凝血功能的药物或化合物研究，为兽医基础研究与生物医学实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：VWF ELISA Kit; F8VWF ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit
缩写：VWF
Uniprot No.：Q28295
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.32 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 101 | ||||
| Range % | 95-107 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 400.0930.1010.097 200.2090.2160.213 100.4220.4410.432 50.7390.7150.727 2.51.1681.1241.146 1.251.6021.7091.656 0.6251.7791.8791.829 02.1702.2972.234 | ||||||
货期：3-5 working days
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