| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHO1; KNSL5; MKLP1; MKLP-1 |
| Entrez GeneID | 9493 |
| WB Predicted band size | Calculated MW: 110 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human MKLP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MKLP1抗体的3篇参考文献，包括研究内容和应用方向：
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1. **文献名称**：*MKLP1. a Chromosomal Passenger, Controls Abscission by Recruiting CEP55 to the Midbody*
**作者**：Gruneberg U, et al.
**摘要**：该研究利用MKLP1抗体进行免疫沉淀和免疫荧光染色，发现MKLP1作为染色体乘客复合体(CPC)的成员，通过结合CEP55蛋白调控胞质分裂的最后阶段(abscission)。实验表明MKLP1的缺失导致CEP55无法定位到中间体(midbody)，从而阻断细胞分裂。
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2. **文献名称**：*The Role of MKLP1 in Spindle Organization and Cytokinesis*
**作者**：Mishima M, et al.
**摘要**：通过MKLP1抗体的免疫印迹和活细胞成像，研究发现MKLP1在维持纺锤体微管稳定性中起关键作用。该蛋白通过调控Aurora B激酶活性，确保姐妹染色体的正确分离和胞质分裂的完成。实验表明MKLP1缺失会导致多核细胞形成。
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3. **文献名称**：*MKLP1 Phosphorylation by Aurora B Triggers Midbody Formation During Mitosis*
**作者**：Zhao WM, et al.
**摘要**：研究利用MKLP1抗体验证其磷酸化修饰对功能的调控。结果显示，Aurora B激酶对MKLP1的磷酸化修饰是中间体形成的关键步骤，这一过程影响细胞分裂的对称性和基因组稳定性。抗体染色显示磷酸化MKLP1在分裂后期富集于中间体区域。
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以上文献均通过MKLP1抗体探究其在细胞分裂中的分子机制，涵盖功能验证、蛋白互作及翻译后修饰等领域。如需具体期刊信息或DOI号，可进一步补充检索条件。
MKLP1 (Mitotic Kinesin-Like Protein 1), also known as KIF23. is a member of the kinesin superfamily of microtubule-associated motor proteins. It plays a critical role in cytokinesis, the final stage of cell division, by facilitating the formation and constriction of the midbody, a transient structure essential for separating daughter cells. Structurally, MKLP1 contains an N-terminal motor domain that binds microtubules and hydrolyzes ATP to generate mechanical force, along with a C-terminal coiled-coil domain involved in protein interactions.
Antibodies targeting MKLP1 are widely used as research tools to study mitotic mechanisms, cell cycle regulation, and cancer biology. These antibodies enable the detection of MKLP1 localization (e.g., at the midbody during cytokinesis) and expression levels in various experimental models, including immunofluorescence, Western blotting, and immunoprecipitation. Dysregulation of MKLP1 has been linked to cytokinesis failure, aneuploidy, and tumor progression, making it a potential biomarker or therapeutic target in cancers. Researchers also utilize MKLP1 antibodies to explore its interactions with regulatory proteins like Aurora B kinase or the chromosomal passenger complex. The specificity and validation of these antibodies (e.g., via knockout cell lines) are critical for ensuring accurate interpretation of experimental results. Overall, MKLP1 antibodies serve as essential reagents for dissecting the molecular machinery of cell division and its implications in health and disease.
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