| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK3; PRKM6; p97MAPK; HsT17250; ERK-3 |
| Entrez GeneID | 5597 |
| WB Predicted band size | Calculated MW: 83 kDa; Observed MW: 105 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human MAPK6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MAPK6抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"ERK3/MAPK6 is required for directed cell migration in cancer cells"*
**作者**：Déléris P. et al.
**摘要**：本研究利用特异性MAPK6抗体，通过Western blot和免疫荧光技术，揭示了MAPK6在癌细胞定向迁移中的关键作用，并发现其亚细胞定位与细胞极性相关。
2. **文献名称**：*"Characterization of a polyclonal antibody to human MAPK6/ERK3"*
**作者**：Soulez M. et al.
**摘要**：该文献报道了一种针对人MAPK6的多克隆抗体的开发与验证，通过免疫沉淀和免疫印迹实验证实其特异性，并用于检测不同组织中的MAPK6表达水平。
3. **文献名称**：*"MAPK6 regulates p53-dependent apoptosis via phosphorylation of Bcl-2"*
**作者**：Coulombe P. et al.
**摘要**：研究使用MAPK6抗体进行免疫共沉淀(Co-IP)和磷酸化分析，发现MAPK6通过磷酸化Bcl-2调控p53依赖性凋亡通路，提示其在肿瘤抑制中的潜在机制。
4. **文献名称**：*"MAPK6 signaling in stress response: Insights from knockout models"*
**作者**：Bogoyevitch M.A. et al.
**摘要**：通过MAPK6抗体检测基因敲除模型中的蛋白表达缺失，该研究揭示了MAPK6在氧化应激反应中的保护性功能，及其与其他MAPK家族成员的协同作用。
这些文献涵盖了抗体在功能研究、信号通路分析及抗体开发中的应用，提供了MAPK6在不同生物学背景下的实验证据。
The MAPK6 antibody is a crucial tool for studying the Mitogen-Activated Protein Kinase 6 (MAPK6), also known as ERK3. a member of the atypical MAPK family. Unlike canonical MAPKs (e.g., ERK1/2), MAPK6 lacks a conserved phosphorylation motif in its activation loop, relying instead on unique regulatory mechanisms, including phosphorylation at serine 189 and interaction with binding partners like MK5. MAPK6 is involved in diverse cellular processes, such as cell cycle progression, migration, inflammatory responses, and stress signaling. Its dysregulation has been implicated in cancer, where it may promote tumorigenesis, metastasis, or chemoresistance in malignancies like lung and breast cancer.
MAPK6 antibodies enable researchers to detect protein expression, localization, and post-translational modifications via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. They are vital for elucidating MAPK6's role in signaling pathways and its crosstalk with other kinases. However, challenges exist in ensuring antibody specificity due to structural similarities among MAPK family members. Validations using knockout controls or siRNA-mediated silencing are essential to confirm target selectivity.
Recent studies highlight MAPK6's potential as a therapeutic target, driving demand for high-quality antibodies. Advances in antibody development, including monoclonal and phospho-specific variants, continue to enhance precision in mapping MAPK6's functional dynamics in health and disease.
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