首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人VGF神经生长因子诱导蛋白(VGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的VGF含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外实验中VGF蛋白表达的动态监测、疾病模型研究或药物干预效果评估，为神经退行性疾病机制探索、代谢综合征相关基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NERP-1 ELISA Kit; NERP-2 ELISA Kit; Nerve growth factor inducible ELISA Kit; Neuroendocrine regulatory peptide-2 ELISA Kit; Neurosecretory protein VGF ELISA Kit; VGF ELISA Kit; VGF_HUMAN ELISA Kit
缩写：VGF
Uniprot No.：O15240
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of human VGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-102 | ||||
| EDTA plasma (n=4) | 86 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1212.0292.0751.976101.6851.7871.7361.63751.3571.4531.4051.3062.51.0681.0461.0570.9581.250.6880.7070.6980.5990.6250.4140.4050.4100.3110.3120.2520.2580.2550.15600.0980.0990.099 | ||||||
货期：3-5 working days
×
