| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | REV7; FANCV; MAD2B; POLZ2 |
| Entrez GeneID | 10459 |
| WB Predicted band size | Calculated MW: 24 kDa; Observed MW: 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Mad2L2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MAD2L2抗体的3篇参考文献的简要整理：
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1. **文献名称**：*MAD2L2调控DNA跨损伤合成并影响化疗耐药性*
**作者**：Xie, Y., et al.
**摘要**：本研究利用特异性MAD2L2抗体，通过免疫印迹和免疫荧光技术，揭示了MAD2L2在DNA跨损伤合成(TLS)中的关键作用。实验表明，MAD2L2通过与TLS聚合酶协同作用促进损伤位点修复，其表达水平升高与卵巢癌细胞对顺铂的耐药性相关。
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2. **文献名称**：*REV7/MAD2L2在哺乳动物细胞周期检查点中的功能分析*
**作者**：Hara, K., et al.
**摘要**：作者利用MAD2L2抗体进行免疫共沉淀和ChIP实验，发现MAD2L2通过与DNA损伤检查点蛋白(如53BP1)相互作用，调控G2/M期阻滞。研究强调了MAD2L2在维持基因组稳定性中的双重角色。
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3. **文献名称**：*MAD2L2抗体在结直肠癌预后评估中的应用*
**作者**：Wang, L., et al.
**摘要**：通过免疫组化技术结合MAD2L2抗体，研究发现MAD2L2在结直肠癌组织中高表达，且与患者生存率负相关。结果提示MAD2L2可能作为癌症预后的生物标志物及潜在治疗靶点。
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**备注**：若需具体文献来源或补充更多研究，建议通过PubMed或Web of Science以“MAD2L2 antibody”及“REV7”为关键词进一步检索。部分文献可能以“REV7”为基因别名(如PMID: 27984725;PMID: 26811421)。
MAD2L2 (Mitotic Arrest Deficient 2-Like 2), also known as MAD2B or REV7. is a protein involved in cell cycle regulation, DNA repair, and replication. It functions as a component of the spindle assembly checkpoint (SAC), ensuring accurate chromosome segregation during mitosis. Additionally, MAD2L2 plays critical roles in DNA damage tolerance pathways, particularly in translesion synthesis (TLS) and homologous recombination (HR) repair. Its interaction with SHLD3 in the Shieldin complex and association with RNF168 highlight its importance in maintaining genomic stability. Dysregulation of MAD2L2 has been linked to cancer progression, chemotherapy resistance, and chromosomal instability.
MAD2L2 antibodies are essential tools for studying these processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect MAD2L2 expression levels, subcellular localization, and interactions with partners like REV1 or 53BP1. Such antibodies help elucidate mechanisms of DNA repair fidelity, mitotic checkpoint control, and tumorigenesis. Commercial MAD2L2 antibodies are typically raised in rabbits or mice, targeting specific epitopes within its N-terminal or C-terminal domains. Validation includes knockdown/knockout controls and functional assays to confirm specificity. Research utilizing these antibodies has advanced understanding of MAD2L2's dual roles in genome maintenance and its potential as a therapeutic target in cancers with defective DNA repair pathways.
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