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产品描述：人血管内皮生长因子受体1(VEGFR-1/Flt1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFR-1/Flt1含量。VEGFR-1/Flt1是一种血管内皮生长因子受体，主要在血管内皮细胞表达，参与血管生成和胚胎发育。其与VEGF-A、PlGF和VEGF-B结合，通过其激酶活性调节血管生成和炎症反应。VEGFR-1/Flt1在多种疾病中起重要作用，是研究血管生成和肿瘤转移的关键靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外实验环境下的基础科研，如探究血管生成相关疾病的分子机制、评估抗血管生成药物的体外效果，或分析不同病理模型中VEGFR-1的表达动态变化；为肿瘤学、心血管研究及炎症相关领域的科研工作者提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 2.7.10.1 ELISA Kit; FLT 1 ELISA Kit; FLT ELISA Kit; Flt-1 ELISA Kit; FLT1 ELISA Kit; Fms like tyrosine kinase 1 ELISA Kit; Fms related tyrosine kinase 1 ELISA Kit; Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) ELISA Kit; Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FRT ELISA Kit; Soluble VEGF receptor 1 14 ELISA Kit; Soluble VEGFR1 variant 2 ELISA Kit; Soluble VEGFR1 variant 21 ELISA Kit; Tyrosine protein kinase FRT ELISA Kit; Tyrosine protein kinase receptor FLT ELISA Kit; Tyrosine-protein kinase FRT ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; Vascular endothelial growth factor vascular permeability factor receptor ELISA Kit; Vascular permeability factor receptor 1 ELISA Kit; Vascular permeability factor receptor ELISA Kit; VEGFR 1 ELISA Kit; VEGFR-1 ELISA Kit; VEGFR1 ELISA Kit; VGFR1_HUMAN ELISA Kit
缩写：FLT1
Uniprot No.：P17948
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-93 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 81-90 | |||||
回收率：
| The recovery of human VEGFR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4862.6982.5922.46051.6851.6671.6761.5442.50.9860.9970.9920.8601.250.6000.6120.6060.4740.6250.3960.3760.3860.2540.3120.2820.2990.2910.1590.1560.1900.1760.1830.05100.1310.1330.132 | ||||||
货期：3-5 working days
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