首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管内皮细胞生长因子C(VEGF-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的VEGFC含量。VEGFC是血管内皮生长因子C，在淋巴管生成和血管生成中发挥关键作用。其背景在于对机体血管和淋巴管发育维持至关重要。研究机制主要是与受体结合，激活下游信号通路，促进内皮细胞增殖、迁移等，在肿瘤淋巴转移等方面研究较多。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于肿瘤微环境分析、转基因动物模型验证、药物干预效果评估等科研领域，为探究VEGF - C在淋巴管相关疾病中的作用机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：VegfcVascular endothelial growth factor C ELISA kit; VEGF-C ELISA kit; Flt4 ligand ELISA kit; Flt4-L ELISA kit; Vascular endothelial growth factor-related protein ELISA kit; VRP ELISA kit
缩写：VEGFC
Uniprot No.：P97953
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.6002.5112.5562.462 502.0202.1522.0861.992 251.3911.3661.3791.285 12.50.7350.7440.7400.646 6.250.3310.3210.3260.232 3.120.2550.2350.2450.151 1.560.1880.1860.1870.093 00.0970.0910.094 | ||||||
货期：3-5 working days
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