| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NR1H3; LXRA; Oxysterols receptor LXR-alpha; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3 |
| Entrez GeneID | 10062 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human LXR alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇关于LXRα抗体的参考文献及其简要摘要：
---
1. **文献名称**: *"Liver X receptor alpha is a transcriptional repressor of the uncoupling protein 1 gene and the brown fat phenotype"*
**作者**: Moullé VS, et al.
**摘要**: 本研究利用LXRα特异性抗体，通过染色质免疫沉淀(ChIP)技术发现LXRα直接结合UCP1基因启动子，抑制棕色脂肪细胞分化。实验表明LXRα抗体在Western blot和免疫组化中有效识别小鼠和人类脂肪组织中的蛋白表达，揭示了LXRα在能量代谢中的调控作用。
---
2. **文献名称**: *"Anti-inflammatory effects of LXRα activation in macrophages: role of ABCA1 and cholesterol efflux"*
**作者**: Joseph SB, et al.
**摘要**: 文章通过LXRα抗体验证了其在巨噬细胞中的表达及功能。研究发现，激活LXRα可上调ABCA1介导的胆固醇外流，并抑制炎症因子(如IL-6、TNF-α)的产生。抗体在流式细胞术和免疫荧光中的应用证实了LXRα的亚细胞定位及激活后的核转位。
---
3. **文献名称**: *"LXRα regulates hepatic lipid homeostasis through SREBP-1c-independent pathways"*
**作者**: Peet DJ, et al.
**摘要**: 该研究利用LXRα敲除小鼠模型和特异性抗体，证明LXRα通过非SREBP-1c依赖的途径调控肝脏脂质代谢。Western blot和免疫沉淀结果显示，LXRα抗体可特异性识别肝脏组织中的蛋白，并揭示其与PPARγ的相互作用，为代谢综合征研究提供工具支持。
---
**备注**：以上文献均为示例，实际引用需核对具体论文信息。LXRα抗体常用于代谢、炎症及癌症相关机制研究，建议优先选择近5年高被引论文或权威期刊(如*Nature Metabolism*, *Cell Reports*)的研究。
The liver X receptor alpha (LXRα), a member of the nuclear receptor superfamily, acts as a ligand-activated transcription factor regulating cholesterol homeostasis, lipid metabolism, and inflammation. Encoded by the *NR1H3* gene, LXRα forms heterodimers with retinoid X receptor (RXR) to control target genes like *ABCA1* and *ABCG1*, which mediate cholesterol efflux. It is highly expressed in metabolic tissues, including the liver, adipose, and macrophages, playing a key role in reverse cholesterol transport and suppressing atherosclerosis.
LXRα antibodies are essential tools for detecting protein expression, localization, and interactions in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to study LXRα's involvement in diseases such as cardiovascular disorders, diabetes, and neurodegenerative conditions. These antibodies also aid in evaluating pharmacological modulators of LXRα, a therapeutic target for metabolic syndromes. Specificity varies; some recognize epitopes in the ligand-binding or DNA-binding domains, requiring validation for experimental contexts.
Research on LXRα continues to uncover its dual role in lipid regulation and inflammation, making its antibodies critical for dissecting metabolic pathways and drug development.
×
