首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管内皮细胞生长因子(VEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFA含量。VEGFA即血管内皮生长因子A，在血管生成、内皮细胞生长等过程中扮演关键角色。其过表达与多种疾病尤其肿瘤的生长、转移密切相关。研究聚焦于抑制VEGFA信号通路，以阻断肿瘤血管生成，从而抑制肿瘤进展，是肿瘤治疗的重要靶点。试剂盒检测范围为3.9 pg/mL-250 pg/mL，适用于心血管疾病模型、肿瘤微环境研究、创伤修复实验等科研领域；为研究血管生成调控机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
缩写：VEGFA
Uniprot No.：P16612
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 pg/mL-250 pg/mL
灵敏度：0.97 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 84-101 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2502.7122.8922.8022.614 1252.3692.3172.3432.155 62.51.5681.6041.5861.398 31.250.8770.8880.8830.695 15.60.5580.5620.5600.372 7.80.3710.3840.3780.190 3.90.2760.2860.2810.093 00.1880.1870.188 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat VEGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled VEGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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