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产品描述：猪血管内皮细胞生长因子(VEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFA含量。VEGFA即血管内皮生长因子A，是一种促进血管生成的重要蛋白。在肿瘤等疾病中，其过度表达会促使肿瘤血管生成以提供养分。研究机制主要围绕抑制VEGFA活性或阻断其信号通路，从而抑制血管生成，为抗癌等治疗提供新思路。试剂盒检测范围为4.69 pg/mL-300 pg/mL，组织修复、炎症反应及肿瘤微环境等研究；体外培养模型、动物实验等科研场景中血管生成相关机制研究；心血管疾病模型、创伤修复研究及药物干预实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：VEGFA ELISA kit; VEGFVascular endothelial growth factor A ELISA kit; VEGF-A ELISA kit; Vascular permeability factor ELISA kit; VPF ELISA kit
缩写：VEGFA
Uniprot No.：P49151
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：4.69 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:10 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:40 | Average % | 86 | ||||
| Range % | 81-92 | |||||
回收率：
| The recovery of pig VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.0321.9842.0081.8711501.7461.7441.7451.608751.4651.4871.4761.33937.51.1091.1271.1180.98118.750.7630.7360.7500.6139.370.5250.5210.5230.3864.690.3340.3480.3410.20400.1350.1380.137 | ||||||
货期：3-5 working days
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