| WB | 1/500-1/1000 | Rat，Hamster |
| IF | 咨询技术 | Rat，Hamster |
| IHC | 咨询技术 | Rat，Hamster |
| ICC | 技术咨询 | Rat，Hamster |
| FCM | 咨询技术 | Rat，Hamster |
| Elisa | 咨询技术 | Rat，Hamster |
| Aliases | MDS; LIS1; LIS2; MDCR; NudF; PAFAH |
| Entrez GeneID | 5048 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat，Hamster |
| Immunogen | A synthetic peptide of human LIS1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇与LIS1抗体相关的研究文献及其摘要概括：
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1. **文献名称**：*Isolation of a Miller-Dieker lissencephaly gene containing G protein β-subunit-like repeats*
**作者**：Reiner, O. et al.
**摘要**：该研究首次克隆了LIS1基因(PAFAH1B1)，发现其与无脑回畸形相关。通过抗体验证，LIS1蛋白在神经元迁移中发挥关键作用，并定位在细胞质及微管相关结构中，为后续研究提供重要抗体工具。
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2. **文献名称**：*Reduced LIS1 expression leads to impaired neuronal migration and epilepsy in a mouse model*
**作者**：Hirotsune, S. et al.
**摘要**：通过构建LIS1杂合缺失小鼠模型，利用LIS1抗体检测蛋白表达水平，发现LIS1表达降低导致皮层神经元迁移缺陷和小脑发育异常，揭示了LIS1剂量敏感性对神经发育的影响。
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3. **文献名称**：*LIS1 and dynein: A shared mechanism for neuronal migration and intracellular transport*
**作者**：Vallee, R.B. et al.
**摘要**：研究通过免疫共沉淀(使用LIS1抗体)证实LIS1与细胞质动力蛋白复合物相互作用，调控微管动态和细胞器运输，为LIS1在神经元迁移及信号转导中的分子机制提供证据。
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4. **文献名称**：*LIS1 regulates extracellular matrix remodeling and neurogenesis in the developing brain*
**作者**：Sapir, T. et al.
**摘要**：利用LIS1抗体进行免疫荧光染色，发现LIS1缺失导致神经前体细胞增殖异常及细胞外基质重塑障碍，提出LIS1通过整合素信号通路协调脑发育的新机制。
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这些文献均涉及LIS1抗体的实验应用(如Western blot、免疫组化等)，并围绕其功能机制或疾病模型展开研究。
The LIS1 antibody targets the LIS1 protein, encoded by the *PAFAH1B1* gene, which plays a critical role in neuronal migration and brain development. LIS1 (lissencephaly-1) is named for its association with lissencephaly ("smooth brain"), a rare neurodevelopmental disorder characterized by impaired cortical layering due to defective neuronal migration during embryogenesis. Mutations in *PAFAH1B1* are linked to Miller-Dieker syndrome, a severe form of lissencephaly accompanied by developmental delay and seizures.
The LIS1 protein interacts with microtubule-associated proteins and molecular motors (e.g., dynein), regulating mitotic spindle orientation, nuclear translocation, and intracellular transport. Antibodies against LIS1 are essential tools for studying these processes in neurodevelopment and disease models. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess LIS1 expression, localization, and interactions in cellular or tissue samples. Researchers also employ LIS1 antibodies to investigate mechanisms underlying neurodevelopmental disorders, screen for pathogenic variants, or validate gene-editing outcomes in experimental systems. Their specificity and reliability are critical for elucidating LIS1's role in both normal brain formation and pathological conditions.
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