首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬血管内皮细胞生长因子(VEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFA含量。VEGFA 即血管内皮生长因子 A，是血管生成过程中的关键因子。它可促进内皮细胞增殖、迁移，增加血管通透性。在肿瘤等疾病中高表达，促使肿瘤血管生成以提供营养。目前有诸多针对它的靶向治疗研究，通过阻断其活性抑制肿瘤生长和转移。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于犬类心血管疾病模型、肿瘤血管化研究、创伤修复实验等基础科研领域，为犬类VEGF相关分子机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：VEGFA ELISA Kit; VEGF ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
缩写：VEGFA
Uniprot No.：Q9MYV3
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-106 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of dog VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-93 | ||||
| EDTA plasma (n=4) | 88 | 86-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2003.0763.0503.0632.9641002.5382.5172.5282.429501.9841.9121.9481.849251.2031.2281.2161.11712.50.6710.6790.6750.5766.250.3080.3170.3130.2143.120.1670.1600.1640.06500.1010.0970.099 | ||||||
货期：3-5 working days
×
