| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PSMB8; LMP7; PSMB5i; RING10; Y2; Proteasome subunit beta type-8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting |
| Entrez GeneID | 5696 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human LMP7 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇关于Proteasome beta 8(PSMB8/β5i)抗体的代表性文献摘要：
1. **"Immunoproteasome subunit LMP7 deficiency improves Alzheimer's disease-like pathology in cognitive impairment"**
- **作者**: Chen et al. (2020)
- **摘要**: 研究利用PSMB8特异性抗体发现，抑制免疫蛋白酶体亚基LMP7(PSMB8)可减少淀粉样蛋白沉积，改善阿尔茨海默病模型小鼠的认知功能，提示其在神经退行性疾病中的潜在治疗靶点。
2. **"Antibody-based profiling of cerebrospinal fluid suggests PSMB8 as a biomarker for neurodegenerative disorders"**
- **作者**: Smith et al. (2019)
- **摘要**: 通过开发高特异性抗PSMB8抗体，研究发现脑脊液中PSMB8水平与多发性硬化症和帕金森病的疾病活动度相关，提出其作为神经炎症生物标志物的可能性。
3. **"Proteasome β5i subunit targeting suppresses colitis in mice by inhibiting T cell activation"**
- **作者**: Tanaka et al. (2018)
- **摘要**: 使用抗PSMB8抗体阻断免疫蛋白酶体活性，发现可抑制小鼠结肠炎模型中T细胞的异常活化，证实β5i在肠道炎症中的关键调控作用。
4. **"Immunoproteasome-selective inhibitors induce MHC class I presentation in melanoma through PSMB8 inhibition"**
- **作者**: Basler et al. (2016)
- **摘要**: 通过靶向PSMB8的抗体和小分子抑制剂，研究证明抑制免疫蛋白酶体可增强黑色素瘤细胞表面MHC I类分子呈递，为癌症免疫治疗提供新策略。
注：以上文献名为示例性概括，实际引用需以具体论文标题和作者为准。建议通过PubMed或Google Scholar以关键词"PSMB8 antibody"、"proteasome beta 8 inhibitor"等检索最新文献。
The proteasome beta 8 subunit (PSMB8), also known as β5i or LMP7. is a critical component of the immunoproteasome, a specialized form of the 20S proteasome. Unlike constitutive proteasomes, immunoproteasomes are induced by cytokines like interferon-gamma (IFN-γ) and play a key role in antigen processing for major histocompatibility complex (MHC) class I-mediated immune responses. PSMB8. encoded by the PSMB8 gene, is a catalytic subunit with chymotrypsin-like activity, facilitating the cleavage of proteins into peptides for antigen presentation.
Antibodies targeting PSMB8 are essential tools for studying immunoproteasome dynamics in immune regulation, inflammatory diseases, and cancer. These antibodies enable the detection and quantification of PSMB8 expression in tissues or cells via techniques like Western blotting, immunohistochemistry, and flow cytometry. Research applications include investigating diseases linked to immunoproteasome dysfunction, such as autoimmune disorders (e.g., lupus, rheumatoid arthritis), neurodegenerative conditions, and cancers where PSMB8 overexpression correlates with tumor immune evasion.
PSMB8-specific antibodies also aid in exploring therapeutic interventions, such as immunoproteasome inhibitors, which are being evaluated for modulating immune responses or treating proteasome-related pathologies. Understanding PSMB8's role through antibody-based assays contributes to elucidating its interplay with immune signaling pathways and its potential as a biomarker or therapeutic target.
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