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产品描述：人血管内皮生长因子121(VEGF121)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的VEGF121含量。VEGF121 是血管内皮生长因子（VEGF）的一种主要亚型。它在血管生成、肿瘤生长和转移等过程中起关键作用。其研究机制主要聚焦于与受体结合，激活下游信号通路，促进血管内皮细胞的增殖、迁移和存活，目前是肿瘤等疾病治疗的重要靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，肿瘤血管生成机制研究、心血管疾病模型评估、再生医学实验等科研场景，体外实验、动物模型及细胞水平的研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：VEGF121
Uniprot No.：P15692
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF121 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 86-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human VEGF121 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-104 | ||||
| EDTA plasma (n=4) | 91 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4152.3142.3652.22251.5321.6331.5831.4402.50.9720.9510.9620.8191.250.5580.5470.5530.4100.6250.4040.4110.4080.2650.3120.2850.2740.2800.1370.1560.2260.2200.2230.08000.1430.1420.143 | ||||||
货期：3-5 working days
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