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产品描述：人血管生成抑制蛋白-1(VASH1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VASH1含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域中对血管生成相关机制的研究，例如肿瘤微环境分析、抗血管生成药物开发中的疗效评估，或心血管疾病模型中血管重塑过程的分子机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：KIAA1036 ELISA Kit; VASH ELISA Kit; VASH1 ELISA Kit; VASH1_HUMAN ELISA Kit; Vasohibin 1 ELISA Kit; Vasohibin-1 ELISA Kit
缩写：VASH1
Uniprot No.：Q7L8A9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VASH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-106 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 82-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of human VASH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-100 | ||||
| EDTA plasma (n=4) | 95 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4662.3432.4052.28020001.5251.5881.5571.43210000.9380.9290.9340.8095000.5500.5620.5560.4312500.3560.3730.3650.2401250.2620.2650.2640.13962.50.1800.1750.1780.05300.1230.1270.125 | ||||||
货期：3-5 working days
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