| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAM; CCM1 |
| Entrez GeneID | 889 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human KRIT1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于KRIT1抗体的3篇参考文献的简要列举：
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1. **文献名称**: *KRIT1 interacts with β1 integrin to promote endothelial cell adhesion and spreading*
**作者**: Glading A, et al.
**摘要**: 本研究通过免疫共沉淀和免疫荧光技术(使用KRIT1特异性抗体)揭示了KRIT1蛋白与β1整合素的直接相互作用，并证明其在调节内皮细胞黏附和迁移中的关键作用，为理解脑动静脉畸形(CCM)的病理机制提供了依据。
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2. **文献名称**: *Loss of KRIT1 leads to impaired endothelial cell polarity and vascular malformations*
**作者**: Stockton RA, et al.
**摘要**: 利用KRIT1抗体进行蛋白质印迹和免疫组化分析，发现KRIT1缺失导致内皮细胞极性异常，进而引发血管结构紊乱。研究强调了KRIT1在维持血管屏障和细胞骨架组织中的功能。
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3. **文献名称**: *KRIT1 regulates the actin cytoskeleton through interaction with Rap1 and microtubules*
**作者**: Liu H, et al.
**摘要**: 通过KRIT1抗体的免疫定位实验，发现KRIT1通过调控Rap1信号通路和微管稳定性影响细胞骨架动态，揭示了其在血管发育及CCM病变中的分子机制。
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如果需要更多文献或具体细节，可进一步补充说明。
The KRIT1 antibody is a crucial tool in studying cerebral cavernous malformations (CCMs), a vascular disorder affecting the central nervous system. KRIT1 (Krev Interaction Trapped 1), encoded by the *CCM1* gene, is a scaffold protein involved in maintaining vascular integrity by regulating endothelial cell signaling and cell-cell junctions. Mutations in *KRIT1* are linked to familial CCMs, characterized by fragile, leaky blood vessels that predispose individuals to seizures, strokes, and neurological deficits.
KRIT1 antibodies are primarily used to detect and quantify KRIT1 protein expression in research settings, including Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate KRIT1's role in pathways like the RhoA/ROCK signaling axis, its interaction with binding partners (e.g., ICAP1α, HEG1), and its involvement in oxidative stress responses. Studies using KRIT1 antibodies have revealed its localization at endothelial adherens junctions and its role in stabilizing vascular structures by inhibiting pro-angiogenic signals.
Additionally, KRIT1 antibodies aid in modeling CCM pathogenesis in vitro and in vivo, facilitating drug discovery for CCMs. Their specificity varies depending on epitope targets (e.g., N-terminal vs. C-terminal regions), and validation is critical due to potential cross-reactivity. Overall, KRIT1 antibodies remain indispensable for advancing our understanding of cerebrovascular biology and CCM-related therapeutic strategies.
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