首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠尿苷二磷酸葡萄糖醛酸转移酶1(UGT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的UGT1A1含量。UGT1A1是重要的药物代谢酶基因，在人体肝脏中表达。它参与多种内源性和外源性物质的葡萄糖醛酸化代谢反应。研究其机制有助于了解药物代谢过程、药物相互作用，对评估药物疗效和不良反应、实现精准用药有重要意义。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，该产品适用于药理学研究、肝脏功能评估、代谢调控机制探索等领域，尤其适合用于研究药物干预、毒素暴露或病理状态下UGT1的活性调控及组织分布特征，为毒理学、药代动力学及代谢性疾病研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ugt1a1; Ugt1; UDP-glucuronosyltransferase 1A1; UGT1A1; B1; UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1
缩写：UGT1A1
Uniprot No.：Q64550
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat UGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 110 | |||||
| Range % | 104-114 | ||||||
回收率：
| The recovery of rat UGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20001.9412.0141.9781.87510001.5031.4981.5011.3985000.9991.0311.0150.9122500.6100.6310.6210.5181250.4170.4070.4120.30962.50.2350.2280.2320.12931.250.1480.1500.1490.04600.1010.1040.103 | |||||||
货期：3-5 working days
×
