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产品描述：小鼠线粒体棕色脂肪解偶联蛋白1(UCP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的UCP1含量。UCP1即解偶联蛋白1，主要存在于棕色脂肪组织中。其背景在于它能使氧化磷酸化解偶联，将能量以热能形式释放。研究机制围绕其产热功能展开，探索通过激活UCP1提高能量消耗，有望为肥胖、糖尿病等代谢疾病治疗提供新途径。试剂盒检测范围为4.68 pg/mL-300 pg/mL，该产品适用于基础科研领域，如探究环境温度变化、药物干预或基因修饰对棕色脂肪活性的影响，亦可应用于代谢性疾病模型中能量代谢通路的机制研究。支持科研人员高效开展能量代谢调控、肥胖机制、糖尿病病理等方向的UCP1蛋白表达水平检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ucp1; Slc25a7; Ucp; Mitochondrial brown fat uncoupling protein 1; UCP 1; Solute carrier family 25 member 7; Thermogenin
缩写：UCP1
Uniprot No.：P12242
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：4.68 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of mouse UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-96 | ||||
| EDTA plasma (n=4) | 102 | 96-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.3882.4662.4272.2671501.7021.7021.7021.542750.9370.9890.9630.80337.50.6410.6360.6390.47918.750.3400.3680.3540.1949.370.2870.2880.2880.1284.680.2280.2110.2200.06000.1530.1660.160 | ||||||
货期：3-5 working days
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