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产品描述：人线粒体棕色脂肪解偶联蛋白1(UCP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的UCP1含量。UCP1 即解偶联蛋白 1，主要存在于棕色脂肪组织中。其背景在于它能使氧化磷酸化解偶联，将能量以热能形式释放。研究机制聚焦于其激活可增加能量消耗、促进脂肪分解，在肥胖、代谢综合征等疾病治疗研究中有重要价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景中UCP1蛋白水平的定量分析，例如通过检测不同生理或病理状态下（如冷暴露、能量限制或代谢干预模型）血清/血浆样本的UCP1动态变化，或分析脂肪组织匀浆中UCP1的表达差异，为探究能量代谢相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：mitochondrial brown fat uncoupling protein ELISA Kit; Mitochondrial brown fat uncoupling protein 1 ELISA Kit; SLC25A7 ELISA Kit; Solute carrier family 25 member 7 ELISA Kit; Thermogenin ELISA Kit; UCP 1 ELISA Kit; UCP ELISA Kit; UCP1 ELISA Kit; UCP1_HUMAN ELISA Kit; uncoupling protein 1 (mitochondrial, proton carrier) ELISA Kit; Uncoupling protein 1 ELISA Kit
缩写：UCP1
Uniprot No.：P25874
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-105 | |||||
回收率：
| The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 92 | 89-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.0931.9522.0231.94010001.3441.3211.3331.2505000.7810.7770.7790.6962500.4710.4550.4630.3801250.2830.2780.2810.19862.50.2120.2070.2100.12731.250.1490.1450.1470.06400.0850.0810.083 | ||||||
货期：3-5 working days
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