首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人尿皮素-3(UCN3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的UCN3含量。UCN3 是重要靶点，它属于促皮质素释放因子家族成员。其在人体能量代谢、应激反应等生理过程中发挥作用。研究机制主要聚焦于它与受体的结合，以及相关信号通路的激活，借此探索在肥胖、糖尿病等疾病治疗中的潜在价值。试剂盒检测范围为6.25 pg/mL-400 pg/mL，可稳定应用于基础科研领域，如探究UCN3在代谢性疾病动物模型中的表达变化、评估药物干预对UCN3信号通路的影响，或通过临床样本分析其与病理状态的相关性，支持实验室常规研究与高通量筛选需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：UCN3 ELISA kit; SPCUrocortin-3 ELISA kit; Stresscopin ELISA kit; Urocortin III ELISA kit; Ucn III ELISA kit
缩写：UCN3
Uniprot No.：Q969E3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human UCN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 98-104 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 101-109 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human UCN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 81-89 | ||||
| EDTA plasma (n=4) | 99 | 96-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.6042.6382.6212.4162002.2842.1142.1991.9941001.5551.5981.5771.372500.9770.9350.9560.751250.5510.5870.5690.36412.50.4050.4320.4190.2146.250.3040.3180.3110.10600.2040.2060.205 | ||||||
货期：3-5 working days
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